ThreatLocker has secured $190 million in Series F funding led by Elephant, with continued support from D. E. Shaw Ventures and Arthur Ventures, and a significant new investment from Koch Disruptive Technologies.
The capital will support continued development of the company’s existing controls for AI-related security risks, further improvements to its Zero Trust Platform, and international expansion, beginning with the opening of a Reading, U.K. office.
The funding comes as organizations face an evolving threat landscape shaped by the need to secure AI agents and defend against AI-accelerated software exploits. ThreatLocker has continued to expand its platform to help secure organizations from both traditional and AI-driven activity. ThreatLocker Allowlisting prevents unauthorized AI tools and AI-generated code from executing, while Ringfencing controls what approved applications and AI agents can access, modify, and interact with.
Additional controls help organizations govern the use of AI websites and protect sensitive information from unauthorized exposure. These innovations build on the recent introduction of ThreatLocker Zero Trust Network Access and Zero Trust Cloud Access, extending the company’s comprehensive Zero Trust Platform across endpoints, networks, and cloud resources from a single dashboard.
“Since our initial investment, ThreatLocker has demonstrated strong execution, established itself as a product leader, and consistently anticipated where the cybersecurity market is headed,” said Jeremiah Daly, partner at Elephant. “As more organizations seek prevention-based security and practical approaches to Zero Trust, ThreatLocker is uniquely positioned to capture that demand. Our continued investment reflects our confidence in both the team and the significant opportunity ahead.”
As part of the investment, Koch Disruptive Technologies will leverage its partner community, industry knowledge, and Koch Labs capabilities to partner with the ThreatLocker team as the company continues to grow.










